Antioxidant Effect of Extra Virgin Olive Oil and Apple Cider Vinegar in Type 2 Diabetic Rat Model

Ruqaiya Nazir; Muniza Saeed; Hifza Noor Lodhi; Ghulam Mustafa; Seher Naeem; Zunnera Rashid

doi:10.37185/LnS.1.1.780

Ruqaiya Nazir Islamic International Medical Collage Rawalpindi, Pakistan
Muniza Saeed Postgraduate Medical Institute Lahore, Pakistan
Hifza Noor Lodhi Rashid Latif Khan University Lahore, Pakistan
Ghulam Mustafa Armed Forces Institute of Urology (AFIU) Rawalpindi, Pakistan
Seher Naeem Combined Military Hospital Rawalpindi (CMH) Lahore, Pakistan
Zunnera Rashid Akhtar Saeed Medical & Dental College Rawalpindi, Pakistan

DOI: https://doi.org/10.37185/LnS.1.1.780

Keywords: Antioxidant Effects, Diabetes Mellitus, Olive Oil.

Abstract

Objective: Comparison between the antioxidant effects of extra virgin olive oil and apple cider vinegar in rats
induced with diabetes mellitus type 2.
Study Design: Randomized controlled trial.
Place and Duration of Study: This Study was conducted in Postgraduate Medical Institute, Animal House,
Lahore, Pakistan from May 2021 to June 2021.
Methods: In this study, 40 male Sprague Dawley rats were divided into 4 groups i.e., Group I was NC (negative
control), Group II PC (positive control), Group III EVOO (Extra virgin olive oil) and Group IV (Apple cider vinegar),
each group having 10 rats. Diabetes was induced in all rats except the rats of NC group at the start of the study by intraperitoneal administration of injection nicotinamide, followed by injection Streptozosin (STZ) after 15
minutes. Group III was given 1ml/100gBW/ day EVOO and Group IV was given 2ml/kg BW/day diluted ACV with
distilled water in 1:5 orally for 4 weeks. Sampling was done after 4 weeks for determination of oxidative stress
markers Malonaldehyde, Superoxide dismutase and Total antioxidant status in serum.
Results: Intake of EVOO and ACV showed reduction in serum Malonaldehyde levels as compared to positive
control group with P-values 0.000 and 0.014 respectively. Serum superoxide dismutase activity and Total
antioxidant status were lowest in the positive control group while both the treatment groups showed
significant enhancement in these parameters as compared to positive control group with P-value = 0.000.
Conclusion: Both extra virgin olive oil and apple cider vinegar have antioxidant effects in type 2 diabetic rats.
However, Extra virgin olive oil is more potent.

How to cite this: Nazir R, Saeed M, Lodhi HN, Mustafa G, Naeem S, Chaudry ZR. Antioxidant Effect of Extra Virgin Olive Oil and Apple Cider Vinegar in Type 2 Diabetic Rat Model. Life and Science. 2024; 5(4): 559-564. doi: http://doi.org/10.37185/LnS.1.1.780