Ameliorative Effects of Grape Seed Proanthocyanidin Extract on Histomorphological Changes in Rat Mandible Induced by Intramuscular Injection of Botox in Masseter

Abstract

Objective: This study was aimed to assess the histomorphological changes in rat mandible induced by injection
botox in masseter muscle and to assess the possible remedial effects of grape seed proanthocyanidin extract
(GSPE) on histomorphology of the rat mandibular condylar cartilage.
Study Design: Laboratory based experimental study.
Place and Duration of Study: This study was conducted at the Department of Anatomy and Pathology, Army
Medical College Rawalpindi, Pakistan from 16th June 2020 to 07th August 2020, with the collaboration of the
Military Hospital, Rawalpindi and the National Institute of Health (NIH), Islamabad.
Methods: Forty, healthy, female (non-pregnant) “Sprague Dawley rats”, 10 weeks of age with weight of 200-
250gm were selected and divided into four groups. Group A control, Groups B, C and D were experimental
groups. Group B was sham injected whilst group C and D were injected by botox in left masseter muscle. Group
D was treated with Grape seed proanthocyanidin extract for 1 month whereas rats from group B and C were
given saline solution as a vehicle. H&E and Toluidine Blue after decalcification of the mandible were used for
staining. Data was analyzed using the SPSS 23. The P-value of ≤0.05 was considered significant. One-way
analysis of variance was applied followed by Tukey's post-Hoc test to seek distinction of quantitative variables
between the groups.
Result: Botox injected group C developed changes when compared with the control group A and sham group B,
including the discontinuation of the condylar cartilage surface and chondrocyte hypertrophy, cluster formation
and disorientation of chondron columns. The experiment did not show any improvement in experimental
group D after treatment with grape seed proanthocyanidin extract.
Conclusion: The administration of grape seed proanthocyanidin extract did not yield the expected
improvements in the histomorphology of the mandibular condylar cartilage.

How to cite this: Iram M, Kiani MRB, Qamar A, Atta MF, Shan M, Zia MS. Ameliorative Effects of Grape Seed Proanthocyanidin Extract on Histomorphological Changes in Rat Mandible Induced by Intramuscular Injection of Botox in Masseter. Life and Science. 2024; 5(4): 482-490. doi: http://doi.org/10.37185/LnS.1.1.766