Variant Analysis of XDR Salmonella Typhi Strains Using Global Alignment Tool Kit in South Asian Region

Abstract

Objective: To concisely compare genomic profiles of XDR Salmonella Typhi isolates from Lahore with
antimicrobial-resistant Salmonella Typhi isolates from other developing nations.
Study Design: Comparative analysis of whole genome sequences.
Place and Duration of Study: The study was conducted from December 2020 to September 2021 at Kauser
Abdulla Malik (KAM) School Life Sciences, Forman Christian College University, Lahore, Pakistan.
Methods: The Galaxy pipeline was run to obtain detailed information at the nucleotide level regarding
mutations that lead to the emergence of XDR strains. Whole genome sequences were analyzed to compare the
genomes of selected three developing nations.
Results: The Pakistani isolates had a significantly higher mutation rate, higher proportion of modifiers, and
silent mutations as compared to isolates of Bangladesh and India.
Conclusion: Cases of Salmonella Typhi XDR are rapidly rising in Asian countries such as Pakistan, Bangladesh,
and India emphasizing the need to analyze and compare its genome with relevant strains. Our study highlights
the unique profile of the Lahore (Pakistan) isolate with the highest mutation rate suggesting the potential
regional differences in selective pressure. Further spotlights the necessity to elucidate the functional
consequences of the identified mutations in S. Typhi isolates.

How to cite this: Niaz M, Mumtaz Z, Ahmed M, Ahmad S, Ali A, Yousaf MZ. Variant Analysis of XDR Salmonella Typhi Strains Using Global Alignment Tool Kit in South Asian Region. Life and Science. 2024; 5(2): 244-250. doi: http://doi.org/10.37185/LnS.1.1.432