The Effects of Alpha Lipoic Acid on Oxidative Stress, Lipid Profile, and Blood Glucose Levels in Type 2 Diabetic Male Sprague Dawley Rats

Abstract

Objective: To determine the effect of Alpha Lipoic Acid (ALA) on blood glucose, lipid profile, and oxidative stress
in streptozotocin-induced type II diabetic male Sprague Dawley rats.
Study Design: Laboratory-based experimental study.
Place and Duration of the Study: The study was carried out at the Physiology Research Lab, Army Medical
College, Rawalpindi, Pakistan, from 20^th  January 2021 to 24^th June 2021.
Methods: Thirty Sprague Dawley (SD) rats were divided into three equal groups. Group I was given a normal
standard rat diet. In Groups II and III after feeding a diet rich in fat and calories for 2 weeks, after feeding a diet
rich in fat and calories for 2 weeks, a single low dose of streptozotocin was injected for Type 2 Diabetes Mellitus
(T2DM) induction. Alpha Lipoic Acid 30 mg/kg body weight/day was administered intraperitoneally in Group III
for 01 weeks after the development of T2DM.
Results: The blood glucose, lipid profile, and serum Malondialdehyde levels were deranged in the diabetic
group. After Alpha Lipoic Acid supplementation, the blood glucose levels, Serum MDA, and levels of
triglycerides, cholesterol, and Low Density Lipoproteins decreased, whereas High Density Lipoproteins level
were significantly raised (p <0.001) in group III as compared to the diabetic group II.
Conclusion: Treatment with Alpha Lipoic Acid reduces oxidative stress and improve reduces oxidative stress
and improves glycemic and lipid profiles in T2DM rats.

How to cite this: khan BU, Arshad S, Ikram F, Yousef I, Naizi I, Ismaeel M. The Effects of Alpha Lipoic Acid on Oxidative Stress, Lipid Profile and Blood Glucose Levels in Type 2 Diabetic Male Sprague Dawley Rats. Life and Science. 2024; 5(1): 89-94. doi: http://doi.org/10.37185/LnS.1.1.405