Protective Effect of Aqueous Neem Leaf Extract on Erythromycin Induced Histomorphological Changes on Hepatocytes of (Albino Wistar) Rats

Abstract

Objective: To determine the protective effect of aqueous Neem leaf extract-induced hepatotoxicity by
Erythromycin.
Study Design: Laboratory-based Experimental study.
Place and Duration of Study: The study was conducted at the Department of Anatomy and Animal House of
Baqai Medical University (BMU) Karachi, Pakistan from January 2019 to March 2019.
Materials and Methods: A total of 80 albino Wistar male rats were randomly divided into 4 groups. Control
group, marked as Group A with no interventions. Only Erythromycin was administered to the rats in group B.
Erythromycin and an aqueous extract of neem
leaves were administered to group C rats. Group D only received an extract of aqueous neem
leaves. Animals' body weight was noted both before and after experiments. Liver weight was also measured
after 14 days when the animals were sacrificed.
Results: Histopathological changes in group B include loss of architecture, portal vein observed with
congestion, and hemorrhages. In Group C, histopathological changes showed hepatocytes with slightly
disturbed and mild congestion with decreased sinusoidal spaces; however, Group A andGroup D observed
normal hepatocyte architecture.
Conclusion: According to the current study, Erythromycin-induced oxidative stress and hepatocyte damage
weremarkedly reduced by aqueous neem leaf extract, which is a powerful antioxidant.