Protective Effect of Pyridoxine on Lead Nitrate Induced Histomorphological Changes in Rat Liver

Pyridoxine on Lead

Abstract

Objective: To study the effects of Pyridoxine on lead nitrate generated histological and morphological changes
in rat liver.
Study Design: It was an experimental study.
Place and Duration of Study: The study was carried out at Anatomy Department of College of Physicians and
rd st Surgeons, Islamabad, Pakistan from 03^rd September, 2019 to 31^st December, 2020.
Materials and Methods: In this study 90 male rats were used and divided into three groups. Control group A
was kept on normal diet. Experimental group B1 was given lead nitrate and B2 was given lead nitrate and
pyridoxine by oral gavage for a period of 2 weeks. All rats were sacrificed after 2 weeks. Results of quantitative
variables such as inflammatory and Kupffer cells were expressed in mean ± S.D. To detect significant difference
among the groups, ANOVA accompanied by Post Hoc Tukey test was applied and qualitative data such as central vein congestion, steatosis, and necrosis were expressed in percentages and Chi Square test.
Results: Exposure to pyridoxine reduces the degenerative changes caused by lead nitrate such as increase in
the number of Kupffer and inflammatory cells. Central vein congestion, steatosis and necrotic foci were also
reduced with a significant p-value.
Conclusion: An ameliorative effect of pyridoxine on the degenerative changes in the liver induced by lead
nitrate was proven through a decrease in the number of kupffer and inflammatory cells. Central vein
congestion, steatosis and necrotic foci were also reduced significantly.

How to cite this: Abbasi S, Khan HM, Khan MY. Protective Effect of Pyridoxine on Lead Nitrate Induced Histomorphological Changes in Rat Liver. Life and Science. 2022; 3(4): 163-167. doi: http://doi.org/10.37185/LnS.1.1.269